Plume Control Using Bioaugmentation with Halorespiring Microorganisms
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J.M. Lendvay, M.J. Barcelona, G. Daniels, M. Dollhopf, B.Z. Fathepure, M. Gebhard, R. Heine, R. Hickey, F. L

Widespread use of chlorinated solvents has resulted in contamination of groundwater requiring effective source and plume containment strategies to prevent contaminant migration and mitigate adverse environmental impacts. The Bachman Road Residential Wells Site is contaminated with predominantly tetrachloroethene and the contaminant plume flows into Lake Huron (USA). Due to natural dechlorination processes, some of the tetrachloroethene has been transformed to cis-1,2-dichloroethene, with lesser amounts of trichloroethene and chloroethene. A bioaugmentation strategy using halorespiring microorganisms at the pilot scale has resulted in complete dechlorination of the groundwater in less than 50 days and significant dechlorination of aquifer solids in less than 80 days. Additionally, terminal restriction fragment length polymorphism analysis has provided evidence that some inoculated microbial species have colonized the test plot and sustained their populations for over four months. This field demonstration illustrates that bioaugmentation using halorespiring microorganisms is a viable alternative for contaminant plume control.

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