In Situ Bioremediation of Chlorinated Solvents in Overburden and Bedrock using Bioaugmentation
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Finn, P.S., A. Kane, J. Vidumsky, D.W. Major, and N. Bauer, 2003, "In Situ Bioremediation of Chlorinated Solvents in Overburden and Bedrock using Bioaugmentation, " Paper A-21, in: V.S. Magar and M.E. Kelley (Eds.), In Situ and On-Site Bioremediation 2003, Proceedings of the Seventh International In Situ and On-Site Bioremediation Symposium (Orlando, FL; June 2003)

The Caldwell Trucking Superfund Site is located in Essex County, NJ, and covers approximately 15 acres. Groundwater contamination involves of chlorinated ethenes and ethanes, impacting both glacial deposits and fractured bedrock, extending approximately 4,000 ft (1200 m) downgradient of the site. TCE concentrations in the source area were as high as 700,000 µg/L (about 60% of TCE solubility). Natural biodegradation is present over much of the site; however some areas, particularly near the source, appeared to be substrate limited. Following microcosm studies which demonstrated that complete degradation of the contaminants could be achieved a comprehensive groundwater remedy was proposed that included bioremediation of the source area. A field pilot test of in-situ enhanced bioremediation in the source area was initiated in 2001. The layout included six nutrient injection wells, screened in glacial deposits and fractured bedrock, and seven monitoring wells. Injection wells were bioaugmented with a culture of naturally occurring microorganisms (KB-1 Culture including Dehalococcoides ethenogenes) in March 2001. In over 20 months of operation, the system was optimized by adjustment of the amendment composition and the injection frequency. Gene probe techniques were used to verify initial and continued survival and propagation of the Dehalococcoides ethenogenes organisms.


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